Biophysical characterisation on a tissue and cellular scale
Caratterizzazione biofisica

Using non-linear optical microscopy techniques (CARS-SHG-TPEF), we study the reprogramming processes of fibroblasts into cardiomyocytes, stem cells' differentiation mechanisms on nano/microstructured polyurethane membranes and the mechanical and morphological properties of fibrin hydrogel scaffolds.

In collaboration with the IRCCS, Istituto Ortopedico Galeazzi in Milan, time-lapse experiments are carried out to study the cellular uptake dynamics of nano-vesicles with anti-inflammatory and regenerative function in cartilage tissue, focusing on human biopsies and bioengineered tissues. To this end, image analysis algorithms are developed to assess the depth of penetration of nano-vesicles, the area and volume occupied and their colocalisation.

Techniques based on atomic force microscopy (AFM) are also implemented to measure and map the mechanical force produced by cellular systems and characterise tumour histological sections and microstructured polymeric materials for applications in regenerative medicine.


Leonardo Mortati